DNA probes testing in Stuat

  DNA Probe Testing

 

DNA probes are small fragment of DNA that help detect the presence of a gene in a long DNA sequence, from a biological system. These DNA probes are prepared for commercial purposes and are said to be the most sophisticated and sensitive means to identify genes or specific DNA sequences. 

DNA probes offer a commercial opportunity for the diagnosis of infection diseases, identification of food contaminants for isolation of genes and in other microbiological tests. It's believed that these DNA probe will be cleaner, simpler, faster and cheaper than the traditional microbiological tests and are also expected to be far more sensitive.

The production of DNA probes can be done by any of the following methods:

  A template DNA with the help of purified biological enzymes.
  DNA probe of specific sequence can also be obtained by using automated DNA synthesizers.
  DNA probe can also be included in viral DNA and may even multiply in bacteria, thus by this way many copies of DNA probe can be obtained.
 

The DNA evaluation consists of the following steps:

  Sample to be tested is treated with detergents and enzymes to remove DNA components.
  Then DNA is then denatured by low PH.
Single stranded DNA binds on filters and is exposed to excess of DNA probes but only one of which will hybridize.
Time unbound DNA is detected by a variety of available methods using florescence and dye etc.

 

 
 
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